THE ACTIVATION OF PAPAIN
نویسندگان
چکیده
منابع مشابه
The Activation of Papain
In previous reports (1, 2) there was presented evidence which could not be fitted into the widely accepted oxidation-reduction theory of papain activation (3). In these experiments it was shown that papain may be inhibited by means of phenylhydrazine and may be reactivated by means of benzaldehyde. In an attempt to study further the process of papain activation, an inactive papain preparation (...
متن کاملStudies on the active--SH group of papain and on the mechanism of papain activation by thiols.
It is now generally agreed that papain possesses a single reactive sulfhydryl group which is essential for activity and which probably participates in the catalytic mechanism by forming acyl intermediates with the different substrates (2, 3). Smith (4) has claimed that the essential sulfhydryl group of papain is kinetically more active than most protein sulfhydryl groups and that it possesses d...
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Papain contains a cysteine-activatable proteinase that hydrolyzes benzoyll-arginlneamide (1). This enzyme has been shown to have a specificity similar to that of crystalline pancreatic trypsin (2) and therefore has been designated papain trypsinase (3). Previous experiments have shown that papain trypsinase exists in two inactive forms which can be designated papain-a-trypsinase and papain-13-t...
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The activation of papain and the inhibition of the active enzyme by carbonyl reagents.
The activation of papain with four different activators is not accompanied by the binding of any of them to the protein. These experiments, taken together with previously reported results, show that the inactive form of papain prepared by the method of Kimmel and Smith (J. Biol. Chem., 207, 5’75 (1954)) is a mixed disulfide formed between the active site sulfhydryl group of the protein and free...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1940
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)73372-3